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KMID : 0359819980270050563
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Journal of Korean Neurosurgical Society
1998 Volume.27 No. 5 p.563 ~ p.569
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Expression of Immediate Early Genes Aaessenger RNAs in Facally Ischemic Rat Brain following Delayed Postischemic Hyperthermia
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Kim Young
Ginsberg Myron D.
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Abstract
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Previous studies have indicated that hyperthemic modulation in the delayed postischmic period. even when occurring days after an ischmic insult. may augment ischemic injury. in order to evaluate the molecular mechanisms of the detrimental effects of hyperthermia on outcome, we performed a study to assess the effects of delayed postischmic hypermia on the regional expression of message for the immediate early genes c-fos and c-jun. 60 minutes of transient middle cerebral artery occlusion was produced in rats by insertion of an intraluminal filament. 24 hours after reperfusion.
rarts which were awake were subjected to normothermic(37-38℃)hyperthermic(40℃) temperature modulation for 3 hours in a heating chamber. Either 2 or 24 hours after temperature modulation. brains were examined for mRNA expression of c-fos and c-jun by the dot blot method. Four regions of interest were chosen : fronto-cingulate, sensorimotor. piriform-insular cortex, and caudoputamen. Dot blot analysis revealed that c-fos mRNA at 2 hours after temperaturemodulation was significantly upregulated in the ipsilateral fronto-cingulate cortex-the site of the ischemicpenumba- in rats exposed to
ischemia＋delayed hyperthemia, in comparison to rats exposed to sham＋ normothermia(one-way ANOVA with post-hoc Bonferroni’s test. p<0.05). in contrast. c-jun mRNA was significantly by ischmiadelayed hyperhermia within regions of core ischemia-i.e., sensorimoter and piriform-insular cortax. and caudoputamen. these finding have extened those of our earlier histopathological study to show that a significant increase in c-fos or c-jun expression may be closely linked to cellilar survival or death after delayed moderate hyperthermia following ischeemia.
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KEYWORD
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Focal ischemia, Hyperthemia, immediate early genes, Rats.,
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